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Cell Culture for Engineers

This course introduces the fundamentals of cell culture techniques for chemical engineers, emphasizing the biotechnological applications in the industry. Students will learn about the principles of cell biology, techniques for maintaining and manipulating cell lines, and the integration of these processes into chemical engineering systems. The outcome is the ability to design, analyze, and optimize bioreactors for cell culture in industrial processes.


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  • Cell Culture
  • Cell Culture Characteristics
  • Type of Plant Tissue Culture
  • Medium of Plant Tissue Culture
  • Advantages and Uses
  • Somatic Embryogenesis
  • Animal Cell Culture
  • Adherent and Non-adherent Cell
  • Term in Animal Cells Culture
  • Biosafety Level and Biosafety Cabinet
  • Cell Counting Technique
  • Population Doubling Level (PDL)
  • Types of Contamination
  • Effect of Contamination
  • Prevention of Contamination
  • Cryopreservation
  • Reactor and Bioreactor
  • Aseptic and Non-aseptic Technique
  • Batch, Continuous, and Semi-Continuous Bioreactor
  • Mass Balances in Batch, Continuous, and Semi-Continuous Bioreactor
  • Lighting, Aeration, and Temperature in Cell Culture

References:

  1. Invitrogen, G. (2014). Cell Culture Basics. Life technologies.
  2. Dodds, J. H., & Roberts, L. W. (1985). Experiments in Plant Tissue Culture. International Potato Center.
  3. Taji, A., Dodd, W. A., & Williams, R. R. (1992). Plant tissue culture practice. University of New England.
  4. R. Ian Freshney (2010). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley-Blackwell.